Preclinical Evaluation of an Innovative Bone Graft of Marine Origin for the Treatment of Critical-Sized Bone Defects in an Animal Model

Autogenous cancellous bone graft is the current gold standard of treatment for management defects since it possesses properties osteoinduction, osteoconduction, and osteogenesis. Xenografts synthetic grafts have been widely reported as available low-cost alternatives, which retain good osteoconductive mechanical properties. Given rich biodiversity ocean organisms, marine sources are particular interest in search alternative with enhanced functionalities. The purpose this paper to assess biocompatibility a marine-derived obtained from shark tooth, an environmentally sustainable abundant raw material fishing. This research presents findings preclinical trial—following UNE-EN ISO 10993—that induced critical-sized defect rabbit model compared results commercial bovine-derived graft. Evaluation by micro-computed tomography histomorphometric analysis 12 weeks after implantation revealed osseointegration, no signs inflammatory foreign body reactions, fibrosis, or necrosis any cases. tooth-derived yielded significantly higher new mineral density values (54 ± 6%) than control (27 8%). Moreover, percentage intersection were much (86 8%) (30 1%) used control. area occupancy tissue test (38 5%) also gave 6%). role physicochemical properties, biphasic structure, composition on stimulation regeneration discussed.